Grupo Dra Lisette Leyton

Referencias Selectas

Ultimos 10 años

1. Ortiz, R., Díaz, J., Díaz, N., Lobos-Gonzalez L, Cárdenas, A., Contreras, P., Díaz, M.I., Otte, E., Cooper-White, J., Torres, V., Leyton, L*. and Quest, A.F.G*. Extracellular matrix-specific caveolin-1 phosphorylation on tyrosine 14 is linked to augmented melanoma metastasis but not tumorigenesis. Oncotarget 7(26), 40571-40593 (2016). *Corresponding authors (equal contribution).
2. Alvarez, A., Lagos-Cabré, R., Kong, M., Cárdenas, A., Burgos-Bravo, F., Schneider, P., Quest, A.F.G., Leyton L. Integrin-mediated transactivation of P2X7R via hemichannel-dependent ATP release stimulates astrocyte migration. BBA-Mol Cell Res. 1863: 2175–2188 (2016)
3. Maldonado, H., Calderon, C., Burgos-Bravo, F., Kobler, O., Zuschratter, W., Ramirez, O., Härtel, S., Schneider, P., Quest, A.F.G., Herrera-Molina, R.*, Leyton, L.*. Astrocyte-to-neuron communication through integrin-engaged Thy-1/CBP/Csk/Src complex triggers neurite retraction via the RhoA/ROCK pathway. BBA-Mol Cell Res. 1864:243-254 (2017). *Corresponding authors (equal contribution).
4. Lagos-Cabré, R., Alvarez, A., Kong, M., Burgos-Bravo, F., Cárdenas, A., Rojas-Mancilla, E., Pérez-Nuñez, R., Herrera-Molina, R., Rojas, F., Schneider, P., Herrera-Marschitz, M., Quest, A. F. G., van Zundert, B. and L Leyton. αVβ3 Integrin regulates astrocyte reactivity. Journal of Neuroinflammation 14:194-206 (2017).
5. Díaz-Valdivia, N.I, Calderon, C.C., Díaz, J., Lobos-Gonzalez L, Sepulveda, H., Ortiz, R., Martinez, S., Silva, V., Maldonado, H.J., Silva, P., Wehinger, S., Burzio, V.A., Torres, V., Montecino, M., Leyton, L.* and Quest, A.F.G*. Anti-neoplastic drugs increase caveolin-1-dependent migration, invasion and metastasis of cancer cells. Oncotarget, 8:111943-111965 (2017). *Corresponding authors (equal contribution).
6. Burgos-Bravo, F., Figueroa, N.L., Casanova-Morales, N., Quest, A.F.G., Wilson, C.A.M.* and Leyton, L.* Single-molecule measurements of the effect of force on Thy-1/αvβ3-integrin interaction using non-purified proteins. Mol Biol Cell. E17-03-0133 (2018). *Corresponding authors (equal contribution).
7. Inostroza-Riquelme, M., Vivanco, A., Lara, P., Guerrero, S., Salas-Huenuleo, E., Chamorro, A., Leyton, L., Bolaños, K., Araya F., E., Quest, A.F.G., Kogan, M. and Oyarzun-Ampuero, F. Encapsulation of Gold Nanostructures and Oil-in-Water Nanocarriers in Microgels with Biomedical Potential. 2018 18;23(5). pii: E1208.
8. R Lagos-Cabré, M Brenet, J Díaz, RD Pérez, LA Pérez, R Herrera-Molina, AFG Quest, L Leyton. Intracellular Ca2+ Increases and Connexin 43 Hemichannel Opening Are Necessary but Not Sufficient for Thy-1-Induced Astrocyte Migration. Int. J. Mol. Sci. 19:2179-2190 (2018).
9. Simón Guerrero, Victor Manuel Díaz-García, Pamela Contreras-Orellana, Pablo Lara, Sujey Palma, Fanny Guzman, Lorena Lobos-Gonzalez, Areli Cárdenas, Ximena Rojas-Silva, Luis Muñoz, Lisette Leyton, Marcelo J Kogan, Andrew FG Quest. Gold nanoparticles as tracking devices to shed light on the role of caveolin-1 in early stages of melanoma metastasis. Nanomedicine 13:1447-1462 (2018).
10. Guerrero S, Inostroza-Riquelme M, Contreras-Orellana P, Diaz-Garcia V, Lara P, Vivanco-Palma A, Cárdenas A, Miranda V, Robert P, Leyton L, Kogan MJ, Quest AFG, Oyarzun-Ampuero F. Curcumin-loaded nanoemulsion: a new safe and effective formulation to prevent tumor reincidence and metastasis. Nanoscale 21;10(47). 22612-22622 (2018).
11. Villamizar-Sarmiento MG, Moreno-Villoslada I, Martínez S, Giacaman A, Miranda V, Vidal A, Orellana SL, Concha M, Pavicic F, Lisoni JG, Leyton L*, Oyarzun-Ampuero FA*. Ionic Nanocomplexes of Hyaluronic Acid and Polyarginine to Form Solid Materials: A Green Methodology to Obtain Sponges with Biomedical Potential. Nanomaterials 9(7). pii: E944 (2019). *Corresponding authors.
12. Arce M, Pinto MP, Galleguillos M, Muñoz C, Lange S, Ramirez C, Erices R, Gonzalez P, Velasquez E, Tempio F, Lopez MN, Salazar-Onfray F, Cautivo K, Kalergis AM, Cruz S, Lladser Á, Lobos-González L, Valenzuela G, Olivares N, Sáez C, Koning T, Sánchez FA, Fuenzalida P, Godoy A, Contreras Orellana P, Leyton L, Lugano R, Dimberg A, Quest AFG, Owen GI. Coagulation Factor Xa Promotes Solid Tumor Growth, Experimental Metastasis and Endothelial Cell Activation. Cancers 11(8). pii: E1103. (2019) doi: 10.3390/cancers11081103.
13. Martínez-Meza S, Díaz J, Sandoval-Bórquez A, Valenzuela-Valderrama M, Díaz-Valdivia N, Rojas-Celis V, Contreras P, Huilcaman R, Ocaranza MP, Chiong M, Leyton L, Lavandero S, Quest AFG. AT2 Receptor Mediated Activation of the Tyrosine Phosphatase PTP1B Blocks Caveolin-1 Enhanced Migration, Invasion and Metastasis of Cancer Cells. Cancers 11(9). pii: E1299. (2019) doi: 10.3390/cancers11091299.
14. Leyton L, Díaz J, Martínez S, Palacios E, Pérez LA and Pérez RD. Thy-1/CD90 a Bidirectional and Lateral Signaling Scaffold. Front. Cell Dev. Biol. 7:132. (2019) doi: 10.3389/fcell.2019.00132
15. Felley-Bosco E, Leyton L. Editorial: Thy1/CD90 Surface Glycoprotein: Sensor of the Microenvironment? Front Cell Dev Biol. 7:162. (2019) doi: 10.3389/fcell.2019.00162. eCollection 2019.
16. Lagos-Cabré, R., Burgos-Bravo, F., Avalos, A.M., Leyton, L. Connexins in Astrocyte Migration. Front Pharmacol. 10:1546 (2020).
17. Díaz-Valdivia, N.I., Díaz, J., Contreras, P., Campos, A., Rojas-Celis, V., Burgos-Ravanal, R.A., Lobos-González, L., Torres, V.A., Perez, V.I., Frei, B., Leyton, L.*, Quest, A.F.G.* The non-receptor tyrosine phosphatase type 14 blocks caveolin-1-enhanced cancer cell metastasis. Oncogene 39 (18): 3693-3709 (2020) https://doi.org/10.1038/s41388-020-1242-3 *Corresponding authors (equal contribution).
18. Rina Ortiz, Jorge Díaz, Natalia Díaz-Valdivia, Samuel Martínez, Layla Simón, Pamela Contreras, Lorena Lobos-González, Simón Guerrero, Lisette Leyton*, Andrew F.G. Quest* (2020) “Src-family kinase inhibitors block early steps of Caveolin-1-enhanced lung metastasis by melanoma cells” Biochemical Pharmacology 177: 113941. *Corresponding authors (equal contribution).
19. Carlos Sanhueza, Jimena Castillo, Manuel Valenzuela-Valderrama, Pamela Contreras, Lorena Lobos-Gonzalez, América Campos, Sergio Wehinger, Alvaro Lladser, Rolf Kiessling, Lisette Leyton*, Andrew Quest *(2020) “Caveolin-1-mediated tumor suppression is linked to reduced HIF1α S-nitrosylation and transcriptional activity in hypoxia” Cancers 12(9), 2349 (2020). *Corresponding authors (equal contribution).
20. Valdivia, A*, Cárdenas, A., Brenet, M., Maldonado, H., Kong. M., Díaz, J., Burridge, K., Schneider, P., San Martín, A., García-Mata, R., Quest, A.F.G., Leyton, L*. “Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells”. Cell Communication and Signaling. 18(1):129 (2020). *Corresponding authors (equal contribution).
21. Burgos-Bravo, F., Martínez-Meza, S., Quest, A.F.G., Wilson, C.A.M. and Leyton, L.¨Application of force to a Syndecan-4 containing complex with Thy-1- αvβ3 Integrin accelerates neurite retraction¨ Frontiers in Molecular Biosciences 7: 254 (2020).
22. Orellana-Serradell, O., Díaz, M.C., González, M.F., Gutiérrez, M., Herrera, D., Jara, D., Maureira, D., Ruiz-Fuentes, J.L., Sanhueza, Leyton, L. “Does Peer Reviewing for COVID-19-Related Papers Still Work?”. Front. Res. Metr. Anal., 08 October 2020.
23. Simon, L., Campos, A., Leyton, L., Quest, A.F.G. “Caveolin-1 function at the plasma membrane and in intracellular compartments in cancer” Cancer Metastasis Rev. 2020; 39(2): 435–453.
24. Brenet M, Martínez S, Pérez-Nuñez R, Pérez LA, Contreras P, Díaz J, Avalos AM, Schneider P, Quest AFG and Leyton L. Thy-1 (CD90)-Induced Metastatic Cancer Cell Migration and Invasion Are β3 Integrin-Dependent and Involve a Ca2+/P2X7 Receptor Signaling Axis. Front. Cell Dev. Biol. 8:592442 (2021). doi: 10.3389/fcell.2020.592442.
25. Leonardo Perez, Aysha Rashid, J. Dale Combs, Pascal Schneider, Andrés Rodríguez, Khalid Salaita, and Lisette Leyton. An Outside-In Switch in Integrin Signaling Caused by Chemical and Mechanical Signals in Reactive Astrocytes. Cell Dev. Biol. 9:712627 (2021).
26. Renato Burgos-Ravanal, América Campos, Magda C Díaz-Vesga, María Fernanda González, Daniela León, Lorena Lobos-González, Lisette Leyton, Marcelo J Kogan, Andrew FG Quest. Extracellular Vesicles as Mediators of Cancer Disease and as Nanosystems in Theranostic Applications. Cancers 13 (13), 3324 (2021).
27. Leonardo Perez, Lisette Leyton*, and Alejandra Valdivia*. Thy-1 (CD90), integrins and syndecan 4 are key regulators of skin wound healing. Front. Cell Dev. Biol. 10:810474. doi: 10.3389/fcell.2022.810474, (2022). *Corresponding authors (equal contribution).
28. Díaz-Valdivia, N., Martinez-Meza, S., Burgos-Ravanal, R., Simón, L., Perez, V.I., Frei, B., Leyton, L.*, Quest, A.F.G*. “Mitochodrial dysfunction and the glycolytic switch induced by caveolin-1 tyrosine-14 phosphorylation in cancer cells promotes ROS-mediated PTP1B inhibition necessary for enhanced cell migration and invasion”. Cancers. 4(12):2862. doi: 10.3390/cancers14122862. (2022). *Corresponding authors (equal contribution).
29. Ping, H.*, Leyton, L.,* J. Hagood* and Barker, T. “Thy-1, an important integrin regulator in the plasma membrane” in “Integrins: key effectors of cell matrix and cell-cell interactions in health and disease”. Front. Cell Dev. Biol. 10:928510. doi: 10.3389/fcell.2022.928510. (2022). *equal contribution.
30. Pérez LA, León J, López J, Rojas D, Reyes M, Contreras P, Quest AFG, Escudero C*, Leyton L*. The GPI-Anchored Protein Thy-1/CD90 Promotes Wound Healing upon Injury to the Skin by Enhancing Skin Perfusion. International Journal of Molecular Sciences. 23(20):12539. https://doi.org/10.3390/ijms232012539. (2022). *Corresponding authors (equal contribution).
31. Ramón Pérez-Núñez, Alejandro Chamorro, María Fernanda González, Pamela Contreras, Rocío Artigas, Alejandro Corvalán, Brigitte van Zundert, Christopher Reyes, Pablo R. Moya, Ana María Avalos, Pascal Schneider, Andrew F.G. Quest y Lisette Leyton. Protein kinase B (AKT) upregulation and Thy-1-αvβ3 integrin-induced phosphorylation of Connexin43 by activated AKT in astrogliosis. J Neuroinflammation. 20:5 (2023).
32. Palacios, E., Lobos, L., Guerrero, S., Heinecke, J., Quest, AFG., Valenzuela*, M., & Leyton*, L. Helicobacterpylori outer membrane vesicles induce astrocyte reactivity through nuclear factor-Kappa B activation and cause neuronal damage in vivo in a murine model. J Neuroinflammation. 20(1):66 (2023). *Autores correspondientes (equal contribution).
33. Horacio Maldonado and Lisette Leyton. CSK-mediated signalling by integrins in cancer. Front. Cell Dev. Biol. 11:1214787 (2023). https://doi.org/10.3389/fcell.2023.1214787
34. Alejandra Valdivia*, Ana María Avalos and Lisette Leyton*. Adhesome complexity and mechanical forces in Thy-1(CD90)-regulated cell adhesion and migration of mesenchymal cells. Front. Cell Dev. Biol. 11:1221306 (2023). *Corresponding authors (equal contribution). https://doi.org/10.3389/fcell.2023.1221306
35. Campos, A., Burgos-Ravanal, R., Huilcamán, R., González, M.F., Díaz, J., Cáceres Verschae, A., Silva, V., Carrasco, M., Guevara, F., Jeldes, E., Varas-Godoy, M., Leyton, L., Lobos-González, L., Quest, A.F.G. “Caveolin-1-dependent inclusion of Tenascin into extracellular vesicles is required to promote malignany in recipient breast cancer cells” Nanomedicine (Lond) 2023 Oct;18(23):1651-1668.
36. Lobos-González, L., Oróstica, L., Díaz-Valdivia, N., Rojas, V.M., Campos, A., Farfan, N., Duran-Jara, E., Leyton, L.*, Quest A.F.G.* “Prostaglandin E2 exposure disrupts E-cadherin/Caveolin-1-mediated tumor suppression to favor Caveolin-1 enhanced migration, invasion and metastasis in melanoma models” Int. J. Mol. Sci. 2023, 24, 16947. *Equal contribution as corresponding authors.
37. Ramón Pérez-Núñez, María Fernanda González, Ana María Avalos y Lisette Leyton. Impacts of PI3K/Protein kinase B (AKT) Pathway Activation in Reactive Astrocytes: From Detrimental Effects to Protective Functions. Neural Regen Res. 2025 Apr 1;20(4):1031-1041. Epub 2024 Jun.
38. Pérez LA. Palacios E, Martínez S, Pérez RD, Díaz J*, and Leyton L*. A Pro-Inflammatory Stimulus versus Extensive Passaging of DITNC1 Astrocyte Cultures as Models to Study Astrogliosis. Int. J. Mol. Sci. 2024, Volume 25, Issue 17, 9454. 2024 August 30. *Equal contribution as corresponding authors
39. Simon, L., Contreras, P., Leyton, L., Quest, A.F.G. “Inhibition of glycolysis and Src/Akt signaling reduces Caveolin-1-enhanced metastasis” Biomed Pharmacother. 176:116841. https://doi.org/10.1016/j.biopha.2024.116841.
40. Huilcaman, R., Campos, A, Orellana, P, Simón, L, Varas-Godoy, M, Grünenwald, F., Shao, B., Heinecke, J., Shao, B., Lobis-González, L., Leyton, L*, Quest, A.F.G*. “Inclusion of Vβ3 integrin into extracellular vesicles in a caveolin-1 tyrosine-14- phosphorylation dependent manner and subsequent transfer to recipient melanoma cells promotes migration, invasion and metastasis” Cell Communication and Signaling. 2025. 23(1):139.
41. Mónica Guzmán-Rodríguez, Tomás Hernández-Díaz, Paula Lisboa, Javier López-Schettini, Sofia Sanhueza, Lisette Leyton, Takao Iwawaki, Ricardo Soto-Rifo, Fabiola Osorio “The IRE1/XBP1s Axis Regulates Innate Immune Responses in Conventional Dendritic Cells During ZIKV Infection”. FASEB J. 39: e71025, 2025. doi: 10.1096/fj.202501186R.
42. Simón, L., Torres, K., Rivera Andrades, G., Arazo-Rusindo. , Mariotti-Celis, M.S., Leyton, L., Andrew F.G. Quest. Brown seaweed extracts inhibit glycolysis and metastasis of cancer cells. J. Functional Foods 2026 139: 107239. https://doi.org/10.1016/j.jff.2026.107239
43. Jorge Díaz, Samuel Martínez, Ignacio Wichman, María Fernanda González, Esteban Palacios, Andrea Tapia Bustos, Mario Herrera-Marschitz, Paola Morales, Tomas Monteverde, Gabriel Diaz-Serrano, Ignacio Leyton-Rivera, Alejandro Chamorro, Rocío Artigas, Alejandro Corvalán, Andrew F. G. Quest, Horacio Maldonado* and Lisette Leyton* “Neuroinflammation-induced downregulation of RAB7 in murine astrocytes disrupts endo-lysosomal homeostasis and induces astrogliosis”. Cell Communication and Signaling 24, 422 (2026). https://doi.org/10.1186/s12964-026-02748-9. *Equal contribution as corresponding authors
44. María Fernanda González, Gabriel Díaz-Serrano, Manuel Valenzuela- Valderrama, Marcelo J. Kogan, Alejandro Corvalán*, Andrew F.G. Quest*, Lisette Leyton* “Circulating Helicobacter pylori Extracellular Vesicles in Serum as Biomarker of Infection”. Frontiers in Cellular and Infection Microbiology, 2026 (Accepted). *Equal contribution as corresponding authors
45. María Fernanda, González, Sebastián Charlin, Gabriel Díaz, Tomás Monteverde, Brian Parra, Rodrigo Naves, Javiera Baeza, Oscar Cerda, Andrew Quest and Lisette Leyton “Helicobacter pylori promote astrogliosis through urease in outer membrane vesicles via TLR2, aggravating symptoms in a preclinical model of multiple sclerosis” 2026 (In preparation).
46. Esteban Palacios-Contreras, Naomi S. Dijksman, Mario Herrera-Marschitz, Bart J.L. Eggen, Lisette Leyton, Wia Baron. “Outer membrane vesicles derived from Helicobacter pylori induce oligodendrocyte-mediated myelin loss” 2026 (In preparation).

Personas

Investigador Principal

Dra. Lisette Leyton

Investigadores Asociados

Andrew FG Quest, Núcleo interdisciplinario de Biología y Genética, ICBM, Facultad de Medicina, Universidad de Chile

Lorena Lobos-González, Núcleo interdisciplinario de Biología y Genética, ICBM, Facultad de Medicina, Universidad de Chile

Colaboradores Nacionales

Miguel O’Ryan (Núcleo interdisciplinario de Microbiología, ICBM, Facultad de Medicina de la Universidad de Chile)

Yalda Lucero (Núcleo interdisciplinario de Microbiología, ICBM, Facultad de Medicina de la Universidad de Chile)

Rodrigo Naves (Núcleo interdisciplinario Inmunología y Farmacología, ICBM, Facultad de Medicina, Universidad de Chile)

Mario Herrera-Marschitz (Núcleo interdisciplinario Inmunología y Farmacología, ICBM, Facultad de Medicina de la Universidad de Chile)

Paola Morales (Núcleo interdisciplinario Inmunología y Farmacología, ICBM, Facultad de Medicina de la Universidad de Chile)

Oscar Cerda, (Núcleo interdisciplinario de Biología y Genética, ICBM, Facultad de Medicina, Universidad de Chile)

Horacio Maldonado-Lorca (Universidad Mayor, Santiago, Chile)

Vicente Torres (Facultad de Odontología, Universidad de Chile, Santiago, Chile)

Montserrat Reyes (Facultad de Odontología, Universidad de Chile., Santiago, Chile)

Marcelo J Kogan (Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile)

Felipe Oyarzun (Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile)

Carolina Valenzuela (Facultad de Veterinario, Universidad de Chile, Santiago, Chile)

Layla Simón (Universidad Finis Terrae, Santiago, Chile)

Denisse Bravo (Universidad Andrés Bello, Santiago, Chile)

Manuel Valenzuela-Valderrama (Universidad Central, Santiago, Chile)

Alejandro Corvalán (Facultad de Medicina, P. Universidad Católica de Chile, Santiago, Chile)

Brigitte van Zundert (Universidad Andrés Bello, Santiago, Chile)

Colaboradores Internacionales

Pascal Schneider, Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland

Rodrigo Herrera-Molina, George Washington University, Washington, D.C., USA

Alejandra D. Valdivia, Emory University, Atlanta, GA, USA

Jay Heinecke, Faculty of Medicine,  University of Washington, Seattle, USA

Steffen Backert, University of Erlangen, Germany

Wia Baron, UMCG, University of Groningen, Groningen, NL

Alumnos de Postdoctorado

María Fernanda González, Ph.D.

Alumnos de Doctorado

Esteban Palacios, Ph.D

Gabriel Diaz Serrano, Ph.D(c)

Sebastián Charlín-Díaz, Ph.D(c)

Ignacio Leyton-Rivera, Ph.D(c)

Apoyo Técnico Profesional

Alejandro Chamorro

Tomás Monteverde

Pamela Contreras

Ruth Mora

Secretaria

Johana Ortiz

Ayudante laboratorio

Rodrigo Arriagada

Líneas de Investigación

Su área de investigación esta enfocada en los mecanismos moleculares de comunicación célula-célula y célula-matriz, señalización celular e interacciones receptor-ligando. Su investigación estudia cómo las células responden a señales del entorno para modificar su estructura, migrar y participar en procesos como reactividad astrocitaria, reparación de heridas, angiogénesis y control de la metástasis. Actualmente investiga las vías de señalización que regulan la comunicación entre astrocitos y neuronas, y entre células tumorales y endotelio. Es autora de más de 100 publicaciones científicas en revistas internacionales ISI Q1.